Pseudotime mapping of Trpc2 mutant (/) and control (/+ littermates) across age. Higher pseudotime values correspond to higher maturity (more similar to adult cell types). Pseudotime is averaged within a cluster; boxplot shows distribution across all cluster averages. P value indicates results from two-sided t -test, mutant versus control cell types. n for increasing age: 82, 137, 131 and 128 cell types for controls, and 119, 135, 129 and 108 cell types for mutants, from n for increasing age of 2, 4, 2 and 2 independent experiments per genotype. Boxplots in b and c show median, interquartile range and 1.5 interquartile range.
