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z -scored calcium traces (mean s.e.m.) aligned to laser onset, showing the effect of optogenetic activation of LHA VGAT + input on the activity of the VGAT + MRN neurons ( n = 5 mice). * P < 0.05; ** P < 0.01; *** P < 0.001. In c , e g , bars indicate median values, error bars are bootstrapped standard error and circles represent individual experimental sessions. See Extended Data Table 1 for statistics.
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