Abundance and KO distribution of functions consistently enriched in the root microbiomes. Boxplots (D) show the cumulative abundance of top 11 abundant bacterial functions that are consistently enriched in roots of wheat, rice, maize, andMedicagocompared with corresponding soils. The distribution (E) of KOs in each function are colored according to their frequency of enrichment (green) or depletion (brown) in the roots of crop species. The colors from light to dark correspond to the number of crop species enriched or depleted, ranging from 0 to 4. The horizontal bars within the boxes represent median values. The tops and bottoms of the boxes represent the 75th and 25th percentiles, respectively. The upper and lower whiskers extend to data no more than 1.5x the interquartile range from the upper edge and lower edge of the box, respectively (root,n= 332; soil,n= 75, adjustedp< 0.05, Wilcoxon rank-sum test). See alsoTable S4J.