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Schematic of study design: we recruited 77 individuals for discovery cohort with either normal glucose tolerance (NGT) (n= 43; 21 male and 22 female) or type 2 diabetes (T2D) (n= 34; 21 male and 13 female), and vastus lateralis muscle biopsies were collected pre and 30 min into an insulin clamp. A validation cohort of 46 individuals with NGT (n= 12; 7 male and 5 female) or T2D (n= 34; 21 male and 13 female) was also recruited.

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