Distance scaling plots for simulated metaphase chromosomes and corresponding experimental data. Simulated chromosomes were sampled as in (C), either 10 times (10- and 100-Mb scales) or 60 times (1-Mb scale) with different starting positions in each of 20 100-Mb simulated chromosomes for representative sampling. Experimental data from chr5, 1 Mb:n= 244 (644) cell (traces), 3 independent experiments; chr2, 10 Mb:n= 187 (346) cell (traces), 2 independent experiments; and chr2q, 100 Mb:n= 212 (686) cell (traces), 3 independent experiments. See alsoFigure S5andVideo S2.