Distance scaling plots for chr5, 1-Mb scale; chr2, 10-Mb scale; and whole chr2 at metaphase. Data from chr5, 1 Mb:n= 400 (824) WT interphase cells (traces) and 244 (644) WT metaphase cells (traces), 3 independent experiments, and 212 (608) ΔSMC4 metaphase cells (traces), 2 independent experiments; chr2, 10 Mb:n= 165 (237) WT interphase cells and 187 (346) WT metaphase cells (traces), 3 independent experiments, and 124 (193) ΔSMC4 metaphase cells (traces), 2 independent experiments; chr2, whole:n= 331 (417) WT interphase cells (traces) and 212 (357) WT metaphase cells (traces), 3 independent experiments, and 206 (355) ΔSMC4 metaphase cells (traces), 2 independent experiments. As the scaling data become very sparse at maximal genomic trace distance, the scaling plots were cropped to 1, 10, and 100 Mb, respectively. See alsoFigure S4.