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3D DNA traces for chr5, 1-Mb scale (12-kb resolution); chr2, 10-Mb scale (200-kb resolution); and whole chr2 (1-Mb resolution) from WT and ΔSMC4 cells at metaphase. Data representative of chr5, 1 Mb: 152 (398) WT cells (traces), 3 independent experiments, and 153 (265) ΔSMC4 cells, 2 independent experiments; chr2, 10 Mb: 159 (286) WT cells (traces), 2 independent experiments, and 106 (153) ΔSMC4 cells (traces), 2 independent experiments; chr2 whole: 130 (212) WT cells (traces), 3 independent experiments, and 51 (62) ΔSMC4 cells (traces), one experiment.
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