Line Plot from Scientific Research

Schematic diagram of a portion of the visual cycle. Extracted whole-eye retinoids were analyzed by HPLC to quantifysyn-11-cis-retinal. The mobile phase for retinal oxime elution was 90% hexanes and 10% ethyl acetate; the mobile phase for the earlier all-trans-retinyl ester elution (not shown in figure) was 99.4% hexanes and 0.6% ethyl acetate. Retinoid levels were determined using a standard curve. Results on the right show median and 95% CI forn= 4 control samples andn= 5 ENVLPE+and eVLP samples. In (D)–(M), injection volumes were adjusted to equalize the injected VLP titers from v3 PE-eVLP and ENVLPE+particles per eye. VLP titers were quantified via ELISA (Figure S15). See alsoFigures S12–S16.

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