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Evaluation of ENVLPE, miniENVLPE, ENVLPE+, and v3b PE-eVLP delivery on several endogenous loci in HEK293T cells (HEK3andRUNX1) and in Neuro-2a cells (Dnmt1) using either the PE2 (default) or PE3 (indicated on top) strategy. Editing efficacy was analyzed via targeted amplicon sequencing, and precision was calculated by the ratio of intended edits divided by the total number of all non-WT reads x 100%. Bars represent mean +/- SD (n= 3 biological replicates, except inHEK3PECsy4ENVLPE [CAG] and PECsy4ENVLPE+[CMV] wheren= 2).HEK3andDnmt1data were analyzed using two-way ANOVA with Bonferroni MCT (****p< 0.0001). The conditions ofRUNX1editing were analyzed by an unpaired two-tailed t test (****p< 0.0001).

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