Comparison of ENVLPE VLPs packaged with different 3'-stabilized PE-RNP complexes comprising the indicated 3'-modifications and covalent or non-covalent Csy4 tethers across a range of VLP doses. PE-mediated reversion of the blue mGL reporter in HEK293T cells is quantified via FACS and displayed as a percentage of green cells (left plot) or the percentage multiplied by the median fluorescence intensity (MFI) of green cells (right plot). PP7, PP7 aptamer bound by PP7 coat protein (PCP); Q1, evopreQ1 pseudoknot; C4, Csy4 aptamer hairpin bound and processed by Csy4; elements are described from 5' to 3' as they appear at the 3'-end of the respective pegRNA. Points represent mean +/- SD (n= 3 biological replicates). Log2(VrelSN): relative transduction volumes in 2x dilution steps. Editing rates of the lowest VLP titer were analyzed via Bonferroni multiple comparisons test (MCT) after one-way ANOVA (****p< 0.0001;**p< 0.01).
