Gel from Scientific Research

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Loading time course of MukBEF6C on negatively supercoiled DNA (pFB527) in the presence of 1 mM ATP and an ATP regeneration system. Reactions were terminated by BMOE crosslinking at the indicated times; samples were denatured by SDS treatment and resolved by agarose gel electrophoresis.
#Gel#Loading Time Course#MukBEF6C#Supercoiled DNA#ATP#ATP Regeneration#BMOE Crosslinking#SDS Treatment#Agarose Gel Electrophoresis
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