A chromatin immunoprecipitation assay was performed using an antibody against NR3C1 on crypts isolated from the WT mice treated with vehicle or CORT for 1 day ( n = 4). IgG was used as a negative control, and the enrichment of NR3C1 binding to the Fkbp5 promoter was quantified using qPCR. The data are presented as the mean SD. The statistical analysis was performed by unpaired two-tailed Student’s t -test for normally distributed data or the MannWhitney test for non - normally distributed data and by one-way ANOVA with Dunnett’s multiple comparisons test or the Kruskal Wallis test with Dunn’s multiple comparisons test for non-normally distributed data. ns, p >= 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001.