Line Plot from Scientific Research

Mutational effects of P2Y2R_Nter3M (Y23 Nter L-R24 Nter D-R26 Nter W triple mutation in P2Y2R) and P2Y4R_Nter3M (L25 Nter Y-D26 Nter R-W28 Nter R triple mutation in P2Y4R) on activation potency for both receptors in response to ATP. This experiment was performed using the G s/q -based cAMP accumulation assay, and a detailed description is provided in Materials and Methods. The difference of luminescence intensity before and after agonist treatment is used to reflect receptor activation and is normalized to the maximal response. The dose-response curse represents the global fit of mean ± SEM from grouped data with three independent experimental replicates (n = 3). A detailed statistical evaluation is listed in Supplementary Table S4.

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