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Dose-response curves for P2Y2R in response to distinct ligands measured by NanoBiT-based G-protein dissociation assay. The data represent mean ± SEM of three independent experimental replicates (n = 3). The fold change in luminescence value after agonist treatment, relative to the basal level, is used to reflect receptor activation and is normalized to the fraction of maximal response. A detailed statistical evaluation is listed in Supplementary Tables S1 and S2.
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