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Reporter (FT promoter: Luc and SV40: Renilla), PIF6αΔC bundle composed of helicesΔC, and phyB Y276H are transfected into the HeLa cells, as indicated by “-” or “+”. The relative firefly luciferase (LUC) activities are normalized to the Renilla luciferase (REN) activity. FT, FLOWERING LOCUS T. Data are presented as means ± SD (n = 3). ** P < 0.01; *** P < 0.001; **** P < 0.0001. PhyB Y276H/Q109A is a mutant hardly interacting with PIF6.

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