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CRISPRCas9-engineered mutant alleles of SCPL25 serine carboxypeptidase orthologues in tomato (Slyc SCPL25 ) and S. prinophyllum (Spri SCPL25 ) (left), along with representative images of transverse fruit sections from mutant plants (right) and quantification of fruit locule number (bottom), showing a consistent increase in fruit locule number across species. n represents the number of fruits counted, represents the average fruit locule number and represents the statistically significant group. Source data and additional statistical information, including P values, are provided in Supplementary Tables 16 and 17 . Scale bars, 1 cm.

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