Gel from Scientific Research

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Reactions set-up as in a , but seeded with 0.8 nM of both strands of one emergent RNA duplex sequence from a – c (Rep(1–4) + with Rep(1–4) − , detected in the sequencing of each product population of the final reactions in b ) as a template, using their constituent triplets as substrates (middle lane: triplets from all four sequences but no template).
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