Workflow from Scientific Research

Open access visualization of Workflow, tCRISPRi Strains, MinCDE Operon, MinD, MinE
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DOI: 10.1038/s41567-025-02878-w

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tCRISPRi strains for gradient repression of the whole minCDE operon (SJ1696) or only minE (SJ1697). These strains allowed us to decrease the Min concentrations diagonally and vertically in the [MinD] versus [MinE] concentration phase diagram (left). [MinD] and [MinE] refer to MinD and MinE protein concentrations, respectively.

#Workflow#tCRISPRi Strains#MinCDE Operon#MinD#MinE#Protein Concentrations#Phase Diagram

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