Left, experimentally determined pattern-formation phase diagram in the [MinD] versus [MinE] space. No patterns formed if either both the MinD and MinE concentrations were low or their ratio was severely disproportionate (grey-shaded areas bounded by dashed lines). In the no-pattern regimes, mini-cells formed when [MinE] [MinD] (red circles; Supplementary Video 4 ), whereas filamentous cells formed when [MinE] [MinD] (blue circles, Supplementary Video 2 ). In the pattern-forming region, we distinguish between normal-sized cells (green-shaded area, green dots) and elongated cells (median length >6 m; blue-shaded region, blue dots). The concentrations of wild-type cells in fast- and slow-growth conditions are marked. Right, kymographs showing the representative dynamics of GFP-MinD in vivo in different regimes. Throughout the paper, we use the following conversion factor from the fold-changes to protein concentrations: [MinD]=[MinE]=2,000 m − 3 in the fast-growth condition (doubling time 25 min).
