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Right, genotypes of the two strains. SJ1935 is a single-inducible strain with the P LTet inducible promoter for gradient expression of minE . SJ1883 is a dual-inducible strain with arabinose-inducible promoter P BAD controlling the expression of minCD and tetracycline-inducible promoter P LTet controlling the expression of minE , independently. Both strains allow the gradient expression of MinE (orange arrows in left panel). The dual-inducible strain additionally allows the independent gradient expression of MinD (green arrows).

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