Line Plot from Scientific Research

Indel formation mediated by WT OrufIscB, OrufIscB-REC and NovaIscB using guide lengths ranging from 12 nt to 28 nt at two target sites. Data are presented as mean s.d.; n = 3 replicate transfections. * P < 0.05; ** P < 0.01; *** P < 0.001 from the two-sided t -test for OrufIscB-REC versus NovaIscB. NS, not significant. For the DYNC1H1 guide, the P values are 0.00002 (14 nt), 0.00005 (15 nt), 0.02642 (16 nt) and 0.01150 (17 nt). For the HPRT1 guide, the P values are 0.00153 (14 nt), 0.00024 (15 nt), 0.06375 (16 nt) and 0.00130 (17 nt).

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