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siRNA transfected HeLa cells were labelled with 25 M BrdU for 24 h, treated with 10 Gy IR for 1 h and then permeablised/fixed 4 h post-irradiation. The intensity of nuclear BrdU staining was quantified in 100 S/G2 cells per experiment. Cells were treated with 50 M mirin 1 h prior to irradiation where indicated. The mean of n = 3 independent experiments is shown (red line).
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