Bar Plot from Scientific Research

siRNA transfected HeLa cells were transfected with the plasmids required to initiate HR at a Cas9-induced DSB located within the LMNA locus. HR (% RFP positive cells with mClover-LMNA staining) was quantified using immunofluorescence. The mean of n = 3 independent experiments is shown with the SEM (red line). A minimum of 500 cells were counted per time point, per experiment. Statistical significance was calculated using: ( b – e ) a Kruskal–Wallis test ([ b ] p = <0.0001, [ c ] p = <0.0001, [ d ] p = <0.0001, [untreated] p = 0.0044, [4 h post-CPT] p = <0.001) and ( f ) an ordinary one-way ANOVA ( p = <0.0001). Source data are provided as a Source Data file.

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