siRNA transfected HeLa cells were labelled with 25 M BrdU for 24 h, treated with 10 Gy IR for 1 h and then permeablised/fixed 4 h post-irradiation. Cells were stained with an anti-BrdU antibody and CENPF (as a marker of S/G2 cells) and the intensity of nuclear BrdU staining was quantified in 100 S/G2 cells per experiment. Cells were treated with 50 M mirin 1 h prior to irradiation where indicated. The mean of n = 3 independent experiments is shown (red line). Statistical significance was calculated using: ( a , b , e ) a Kruskal–Wallis test ([ a ] p = 0.0034, [ b ] p = <0.0001, [ e ] p = <0.0001) and ( c , d ) an unpaired Students t -test (two-sided, equal variance). Source data are provided as a Source Data file.
