Scatter Plot from Scientific Research

Complemented DIAPH1-P1 fibroblasts were treated with 100 nM CPT for 1 h, 30 min into treatment cells were exposed to 10 M EdU for 30 min and then fixed/permeabilised 1 h and 4 h post-treatment. Cells were stained with the antibodies indicated and protein localisation visualised by STORM microscopy. Quantification of the average local density of MRE11, BRCA1 and RPA2 detected around individual EdU molecules within a region of interest (ROI) was carried out and normalised to the untreated controls. A minimum of 100 ROIs were quantified over three independent experiments. The median of n = 3 independent experiments is shown (red line). Representative images of the cells treated with CPT are shown. The scale bar represents 1500 nm.

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