Scatter Plot from Scientific Research

Complemented DIAPH1-P1 fibroblasts were treated with 100 nM CPT for 1 h and then fixed/permeabilised 1 h and 4 h post-treatment. Cells were stained with antibodies to RPA2 in conjunction with CENPF/mitosin as a marker of S/G2 cells. RPA2 fluorescence intensity were quantified in a minimum of 100 S/G2 cells per timepoint, per experiment. The mean of n = 3 independent experiments with the SEM is shown (red line). Statistical significance was calculated using a Kruskal–Wallis test ([ e ] p = <0.0001).

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