Workflow from Scientific Research

The TOP2 reaction cycle is inhibited by the catalytic inhibitors topobexin (9) (TOP2B) or dexrazoxane prior to ATP hydrolysis, thus preventing the cleavage complex from forming, and trapping TOP2 in a closed-clamp conformation on DNA. TOP2 poisons anthracyclines (daunorubicin) and etoposide block the religation step. Catalytic inhibitors block the TOP2 reaction cycle prior to cleavage, and thus fewer TOP2 cleavage complexes are available for poisoning.

DOI