Microscopy from Scientific Research

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Live-cell imaging of NVCM cells in the schedule corresponding to the cytotoxicity/protection experiments (48 h after DAU addition). Changes in morphology are shown in DIC mode while fluorescence signal from JC-1 probe corresponds to the state of mitochondria. Red signal represents actively respiring mitochondria while green indicates mitochondrial depolarization. Scale bar = 100 m. For all box and whisker plots, center line represents the median, + represents the mean. Bounds of box indicate 25th to 75th percentile, whiskers indicate minimal and maximal value. Source data are provided as a Source Data file. Precise n and P values for each experiment are included in Supplementary Data Table 5.
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