A high-speed dark-field microscopy image (exposure time 193 s) of a reactivated axoneme with an attached GNP (scale bar 1 m; right panel: zoom-in). We fit a Gaussian model describing the intensity profile of the GNP and the nearby axoneme ( Methods and Supplementary Fig. 9 ) to precisely measure the centre line (blue) and the GNP position (red cross) to calculate their projected distance d C in the 2D image (pixel size 73 nm).
