Cell uptake for siRNA (blue) and pDNA (orange) determined by flow cytometry at 3 h post-transfection with SaBeH at CR2 (green) and CR4 (blue) and LF (grey). Data are presented as the (upper) and median (lower) fluorescence with standard deviations for three independent biological replicates ( n = 3): siRNA/mean x 10 4 (CR2: 61.7 6.8; CR4: 97.9 16.9; LF: 33.3 1.7); DNA/mean x 10 4 (CR2: 94.4 3.9; CR4: 75.8 6.3; LF: 6.1 0.4); siRNA/median x 10 4 (CR2: 54.4 7.4; CR4: 87.4 14.5; LF: 13.5 0.3); DNA/median x 10 4 (CR2: 78.2 4.8; CR4: 67.2 5.6; LF: 4.4 0.3). Solid horizontal lines denote median, and upper and lower edges correspond to 75th and 25th percentiles, respectively. According to one-sided ANOVA tests, the counts of transfected HeLa cells for siRNA and pDNA when complexed with LF were significantly lower than those complexed with SaBeH. Statistically significant differences are represented with ** for p < 0.01: mean fluorescence siRNA ( p = 0.0009), mean fluorescence DNA ( p = 6.37 x 10 -7 ), median fluorescence siRNA ( p = 0.00023), median fluorescence DNA ( p = 1.5 x 10 - 6 ).
