Microscopy from Scientific Research

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Differentiation was induced in pituispheres from P5 (top) or 7-week-(bottom) old male pituitaries. The presence of differentiated cells within spheres was evaluated by immunofluorescence using LH or collectively GH, PRL and TSH antibodies for POU1F1 lineage cell detection. SOX2 staining confirms the presence of pituisphere forming-cells.
#Microscopy#Pituispheres#Pituitary#Immunofluorescence#Differentiated Cells#LH#GH#PRL#TSH#POU1F1 Lineage Cells#SOX2#Pituisphere Forming-Cells
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