Top left, phylogenetic tree showing the taxon sampling in this study, along with the number of profiled species per clade. Top right and below, Micro-C interaction maps of specific genomic regions (S. arctica, chr. 2: 3400000-3700000, bin 1 kb; C. owczarzaki, chr. 01: 3660000-3800000, bin 400 bp; S. rosetta, chr. 21: 800000-1100000, bin 800 bp; M. leidyi, chr. 8: 15500000-15700000, bin 400 bp; E. muelleri, Emue22: 2200000-2400000, bin 800 bp; T. adhaerens, TadhH1_4: 3880000-4180000, bin 800 bp; N. vectensis, NC_064040.1: 11650000-12000000, bin 1 kb; D. melanogaster, chr. 3L: 20480000-20820000, bin 800 bp; and H. sapiens, chr. 12: 69000000-71000000, bin 5 kb), showing examples of insulation boundaries or chromatin loops. All interaction maps were balanced using ICE normalization.
