Line Plot from Scientific Research

Single-cell lineage from 53BP1PCNA U-2 OS cells overexpressing HRAS. PCNA foci, 53BP1 foci and area of the nucleus over 70 h of live imaging are depicted. Sequential staining intensities from individual cells of the lineage and mean intensities from the corresponding reference populations are depicted for the markers pRb, H2AX, p21 and p53. The total DAPI intensity per nucleus from the corresponding reference populations is depicted as well as from the single cells at the end of live imaging. Representative images of the polyploid cells are included as well as their position within the DAPI scatter plot of the G1 and G2 populations. Scale bars, 10 m. The box plots show the IQR (box limits), with median (centre line) and the whiskers define the lower and upper adjacent value; dots show outliers greater than Q3 + 1.5 IQR. Avg., average.

DOI