General gating strategy for tumor dissociates. Left to right, a viability gate to exclude dead cells, a time gate to exclude electronic noise, a singlet gate to exclude doublets and tumor and immune fraction gates to define the cells of interest. Tumor cells (SOX2 + ) were further gated for the GFAP + fraction and analyzed for MHC class I (HLA-A, HLA-B, HLA-C) and MHC class II (HLA-DR, HLA-DP, HLA-DQ) expression. The numbers indicate the percentage of cells in the respective gates. T cell analysis is shown for comparison.
