Schematic illustration of the eloF locus and the long transgenic reporter constructs containing the entire eloF locus including flanking sequences. Dpro eloF WT (l) and Dcar eloF WT (l) carry wild-type eloF loci from D. prolongata and D. carrolli, respectively. The other two constructs were made by removing the honghaier TE insertion from the D. prolongata sequence (Dpro eloF WT (l) -TE) or adding the D. prolongata honghaier insertion to the D. carrolli sequence (Dcar eloF WT (l) + TE). The eloF locus is placed into the pGreenFriend vector in the forward orientation, so that eloF CDS is transcribed in the same direction as the GFP reporter while the honghaier insertion is in the opposite direction.
