All 5 elongases show a concerted expression increase in D. prolongata males. Dot plots showing normalized expression levels of each gene (RNA-seq data in log 2 cpm). For each group, four biological replicates are represented by jitter points, color-coded by species. Males are in filled symbols; females are in open symbols. *** P < 0.001, ** P < 0.01, * P < 0.05. The structure of the ~ 14 kb genomic neighborhood is displayed on top. Numbers above the consensus sequence constructed from the reference genomes of D. prolongata and D. carrolli are coordinates showing the alignment length. Feature annotations are shown with green boxes representing genes, yellow boxes representing CDS, and the orange box representing a predicted ORF in the honghaier insertion, a TE-like repetitive sequence colored in red. The direction of all features is indicated.
