Expression of genes involved in inflammation (IL-1, IL-6, IL-8, IL-18, MCP-1, TGF- and TNF-) in (i-vii) Chub-S7 cell line ( n =6) and (i-vii) primary Sc-adipocytes ( n =3 per BMI group - subjects with lean, overweight, obesity, morbid obesity), respectively. The Chub-S7 cells were seeded and differentiated with different concentrations of B12 (500nM, 1nM, 100pM, 25pM), and gene expression were measured by RT-qPCR and normalized to 18s rRNA. The primary adipocytes were derived from subcutaneous depots, which were seeded and differentiated with two different concentrations of B12 (500nM, 25pM). The gene expression normalized to L19 were measured by RT-qPCR. Dots depict individual study subjects and data expressed as mean SEM and significance levels indicated as follows; * p <0.05, ** p <0.01, ** p <0.001 compared to control B12 (500nM)
