Bandpass filtered cosine distances of latent space embeddings of untreated mitochondria to the first frame’s mitochondrial latent space embeddings (left) or of ionomycin-treated mitochondria to the first pre-treatment frame’s mitochondrial latent space embeddings (middle and right). Scale bars, 50 m for the full-field image and 10 m in length for the zoomed-in images. Images represent a typical U2OS cell, with two independent timelapses of fields of individual cells run as the experimental sample size.