Scatter plot of cell type proportions estimated by RNAScope/IF ( x -axis) vs. the predicted cell type proportions by the deconvolution methods, for 1vALL top25 marker genes and the full set of common genes. See Additional file 1: Fig. S24 for the Mean Ratio MAD3 or MeanRatio over2 results. Points are colored by the cell type and shaped by the combination of bulk RNA-seq RNA extraction method and library type. The annotation lists the overall Pearson’s correlation (cor) and root mean squared error (rmse).
