Jurkat T cells were infected with WT pNL4.3 HIV particles or Rev-mutated pNL4.3 HIV particles and collected after 3 days and used for ( h ) RNA immunoprecipitation using anti-MTR4 and anti-IgG isotype antibodies. RNA was purified from IPs and subjected to RT-qPCR targeting RRE-containing HIV-1 RNA. All results are shown as mean values ± SEM from n = 5 ( a ), n = 7 ( b ), n = 4 ( c , d , f , g ), n = 6 ( e ), n = 3 ( h ) biological replicates in duplicates. Statistics were performed using a two-sided Wilcoxon signed-rank test for HIV integration ( a ), a two-way ANOVA test ( b - g ), and a two-tailed unpaired Student’s t test ( h ). Significant p values are indicated by the asterisks above the graphs p ≤ 0.05 [*], p ≤ 0.01 [**], ≤0.001 [***], ≤0.0001 [****]. Source data are provided as a Source Data file.
