Colocalization between MATR3-HIV-1 RNA, MTR4-HIV-1 RNA, MATR3-MTR4, and MATR3-MTR4-HIV-1 RNA was quantified by counting the number of colocalizing spots and their volumes from z-stacks obtained from 10 images/biological repetition, n = 3. Results are presented as box and whiskers with 5–95% confidence interval. Median value is shown as a bar, dots are points outside whiskers representing outliers, mean value is shown as “+”. Statistics were performed using a two-tailed unpaired Student’s t test. Statistical comparisons are indicated if p ≤ 0.01 (**), p ≤ 0.001 (***), and p ≤ 0.0001 (****), “ns” indicate no significance.
