Workflow from Scientific Research

Schematic workflow of MS-based AfBPP. Cells are incubated with affinity probe or vehicle control and subsequently irradiated with UV light to covalently link potential target proteins. After lysis, biotin is appended to the alkyne handle via CuAAC chemistry and engaged proteins enriched on avidin beads. Following tryptic digest, the resulting peptides are measured via LCMS/MS and raw data analyzed to infer potential protein targets of the probe.

DOI