Microscopy from Scientific Research

J-Lat 9.2 cells were stimulated with TNF [10 ng/ml] and subjected to RNA FISH and immunostaining using antibodies against MTR4 and MATR3 for subsequent confocal microscopy analysis. MATR3 is shown in green, MTR4 in red, gag HIV-1 RNA in purple, and DAPI-stained nucleus in blue. Bright, large gag HIV-1 RNA spot corresponds to viral transcription site as described previously. White arrows indicate triple colocalization sites. Scale bar = 10 m.

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