SPR and FP data for 2014. Left: SPR sensograms for peptide binding to immobilized FAK FAT, performed at peptide concentrations from 200 M to 49 nM, with thick lines as response data and orange lines representing the kinetic fit. All peptides fit to a pseudo first-order one-site binding model to calculate the K D from kinetic data, other than peptide 1907, which fit to a two-site binding model. The equilibrium fit model is shown in the inset. For 2014, the K D reported is the mean SD of 4 biological replicates ( n = 4). Middle: FP competition assays using TAMRA-paxillin LD2 L10D and recombinant FAK FAT were performed at peptide concentrations from 1.25 mM to 1.22 M, except for 1907, which was performed from 312.5 M to 305.2 nM. The K i reported is the mean SD of 3 biological replicates ( n = 3). Right: The selectivity between wild-type FAT and a FAT mutant (I936E/L994E) demonstrates the peptide’s specific binding to the FAT domain. The source data are provided as a Source Data file.
