Microscopy from Scientific Research

2D classes show the dome-like indentation in the TM domain of YbiO and the change in the curvature of the TM domain. Classes showing different spacing between opposite sides of the TM domain are highlighted with red squares. These 2D classes are taken from a different data collection than the 3D reconstruction reported here. For this alternative data collection, YbiO was solubilized in LMNG/CHS (0.007%/0.0007%) detergent.

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