Ferroptosis inhibitor Liproxstatin-1 (Lip-1) counteract SM effect on sphere formation. After 72 h of pretreatment in adherent condition with Lip-1 (1 M) or Fer-1 (10 M), cells were passaged in suspension culture with increasing concentrations of SM. Results are expressed in fold change compared to control.n= 3 biological replicates. Mean SEM, *pvalue < 0.05, **pvalue < 0.01, ***pvalue < 0.001. Two-way ANOVA followed by multiple comparisons.
