Gel from Scientific Research

Structure-based engineering of REV7 mutants with altered ligand-binding proteins. Interactions between wild-type REV7 and REV7 mutants with REV3 or CHAMP1 were determined by co-immunoprecipitation analysis in HEK293T cells transiently expressing the indicated FLAG or HA-tagged proteins. aa, amino acids; IP, immunoprecipitation; IB, immunoblotting; t, tagged; e, endogenous; WT, wild-type. RD, YAK, L186E, and YKK mutants are defined in the main text.

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