Retinosphere containing CMZ isolated at 137 days gestation and cultured for 84 days, equivalent to 221 days of gestational development. EdU was added on day 214 until fixing. Scale bars, 50 μm. Immunostaining for NR2E3 (yellow) and EdU (magenta). Arrows indicate EdU-labeled NR2E3-positive rod, born between day 214 and day 221 equivalent gestation.
