Heatmap showing germline-specific and soma-expressed piRNA pathway genes ranked by gene expression fold enrichments in the fluorescence-activated cell-sorted germline cells (vas-GFP+) compared to somatic cells (vas-GFP-) dissociated from fly ovaries (*p< 0.01, DEseq2, RNA-seqn= 3 replicates from distinct samples). Normalized RNA-seq counts (DEseq2) from OSCs, vas-GFP+ germline cells, and vas-GFP- somatic cells are also shown. The presence of Tj motifs within ovary ATAC-seq peaks and Tj ChIP-seq peaks (ENCODE, female whole fly) within ±500 bp of a gene’s TSS is depicted with dots.
