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MCF7 cells treated with MEN (10 M) and iPRMT1 (1 M) alone or in combination were cultured in normoxia (20% O2) or hypoxia (1% O2) for 3 h, followed by chromatin immunoprecipitation (ChIP) with anti-HIF2(C) or anti-HIF1 (D) antibody and then RT-qPCR analysis with primers specifically targeting HRE regions in PKM and LDHA promoters (mean SEM,*p< 0.05,**p< 0.01,***p< 0.001).

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